Otoimmün Oral-Dental Ve Diski Örneklerinde Bacteroides Spp. Prevalansi

Abstract

Oral dental ve diski örneklerinde Bacteroides prevalansini belirlemek amaciyla yapilan bu çalismada 420 degisik klinik örnekden toplam 35 Bacteriodes izole edildi. 35 izolatin 14(%40)’ü tür seviyesinde tanimlanirken, 21(%60) izolatin cins seviyesinde muhtemel identifikasyonu gerçeklestirildi. En fazla bakteri izolasyonu oral-dental örneklerden elde edildi. Oral-dental örneklerden elde edilen 8 izolatin 4’ü B.capillosus, 1’i B.eggerthii, 1’i B.distasonis, 1’i B.ruminicola subsp.brevis ve 1’i non-fermentatif Bacteroides grubu olarak tanimlanmistir. Diski örneginden 6 izolat elde edilmis olup, bunlardan 2’si B.multiacidus, 1’i B.capillosus, 1’i B.fragilis, 1’i B.uniformis ve 1’i B.thetaiotaomicron olarak tanimlandi. Cins seviyesinde tanimlanan 21 izolatin 20’si oral-dental apse ve 1’i diski örneginden elde edilmistir. Izolasyon amaciyla Bacteroides’lerin üremesini kolaylastirici Bacteroides Bile Esculin agar ve Streptomisin- Vankomisin kanli agar besiyerleri kullanilmistir.

In this research which was done for the purpose of determination of the prevalance of Bacteroides spp.in oraldental and Stool Samples, totally 35 Bacteroides from 420 different clinical samples were isolated. While 14 isolated Bacteroides out of 35(40%) were identified at species level, the presumptive identification of 21 isolated Bacteroides (60%) was performed at genus level. Most of the isolated bacteria were obtained from the oral-dental samples. Eight of these isolated bacteria obtained from the oral-dental samples were identified such as 4 of them B.capillosus, 1 B.eggerthii, 1 B.distasonis, 1 B.ruminicola subsp.brevis and 1 non-fermentative Bacteroides group. Six of these isolated bacteria obtained from the stool sample have been so found that 2 of them were indentified as B.multiacidus, 1 B.capillosus, 1 B.fragilis, 1 B.uniformis and 1 B.thetaiotaomicron, which were obtained from the stool samples, were identified such as 2 B.multiacidus, 1 B.capillous, 1 B.fragilis, 1 B.uniformis and 1 B.thetaiotaomicron. 20 of the 21 isolated Bacteroides had been obtained from the oral-dental abscess and 1 from the stool sample. For isolation purposes Bacteroides Bile Esculin Agar and Streptomycin-Vankomisin Blood Agar media, which make the reproduction of the Bacteroides easier have been used.