The role of Crocin in an acrylamide-induced neurotoxicity model in Wistar rats

Abstract

Abstract:Aim: The changes in rat brain tissues treated with Crocin (Cr) as a protective agent in an acrylamide (AA) neurotoxicity model wereinvestigated.Material and Methods: The present with of the experimental animal ethics committee at Inonu University, Faculty of Medicine (2016/ A-59). Forty male rats were randomly divided into 4 groups with study was conducted the approval equal number of rats (10):Control, Cr, AA, Cr + AA Groups. Malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant status (TAS), total oxidantstatus (TOS), Oxidative stress index (OSI), superoxide dismutase (SOD), catalase (CAT) and protein values were examined in thebrain tissues.Results: MDA, TOS and OSI levels increased in brain tissues of AA administered rats when compared to the other groups, whilethe GSH, TAS, SOD and CAT levels decreased in the group (p < 0.05). GSH, TAS, SOD and CAT levels increased, but MDA, TOS andOSI levels decreased in the AA + Cr administered group when compared to the AA group (p < 0.05). It was observed that oral AAadministration altered the antioxidant/oxidant balance favoring the oxidants in male rat brain tissues, leading to oxidative stressinduced neurotoxicity, while Cr administration reestablished the normal antioxidant/oxidant balance, preventing the oxidative stressinduced neurotoxicity via detoxification.Conclusion: The present study concluded that the administered Cr dose was sufficient to prevent neurotoxicity and we recommendthat adequate amounts of Cr should be consumed to prevent AA-induced toxicity and improve antioxidant capacity.