dc.contributor.author |
Vardi, N |
|
dc.contributor.author |
Parlakpinar, H |
|
dc.contributor.author |
Ates, B |
|
dc.contributor.author |
Cetin, A |
|
dc.contributor.author |
Otlu, A |
|
dc.date.accessioned |
2022-03-28T11:41:35Z |
|
dc.date.available |
2022-03-28T11:41:35Z |
|
dc.date.issued |
2009 |
|
dc.identifier.uri |
http://hdl.handle.net/11616/58038 |
|
dc.description.abstract |
Objective: To investigate the effect of beta-carotene against testicular injury induced by methotrexate (MTX). |
|
dc.description.abstract |
Design: Experimental study. |
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dc.description.abstract |
Setting: Animal and histology laboratory at Inonu University. |
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dc.description.abstract |
Animal(s): Twenty-eight Wistar male rats. |
|
dc.description.abstract |
Intervention(s): Twenty-eight rats were separated into four groups: control, beta-carotene, MTX, and beta-carotene + MTX. At the end of the treatment, the animals were killed, and tissue samples were examined via histologic and biochemical methods. |
|
dc.description.abstract |
Main Outcome Measure(s): in each group, 100 tubules were classified as intact, sloughing, atrophic, and degenerated. Caspase-3, a universal effector of apoptosis, was evaluated according to staining in place of coloring as weak, mild, and strong. |
|
dc.description.abstract |
Result(s): In the MTX group, 58.5 + 3.7% of tubules were sloughing, 10.8 + 2.1% of tubules were atrophic, and 2.0 + 0.6% of tubules were degenerative. In the beta-carotene + MTX group, the affected tubule number was statistically significantly lower than in the MTX group. The distribution of caspase-3 in the MTX group showed a statistically significant increase, but it decreased in the beta-carotene + MTX group. The enzyme activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GP-x) and the level of malondialdehyde (MDA) increased and decreased in parallel. |
|
dc.description.abstract |
Conclusion(s): Our results indicate that beta-carotene may be useful in decreasing the side effects of chemotherapy, including apoptotic cell death. (Fertil Steril(R) 2009;92:2028-33. (C)2009 by American Society for Reproductive Medicine.) |
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dc.source |
FERTILITY AND STERILITY |
|
dc.title |
Antiapoptotic and antioxidant effects of beta-carotene against |
|
dc.title |
methotrexate-induced testicular injury |
|