dc.contributor.author |
Otlu, B |
|
dc.contributor.author |
Bayindir, Y |
|
dc.contributor.author |
Ozdemir, F |
|
dc.contributor.author |
Ince, V |
|
dc.contributor.author |
Cuglan, S |
|
dc.contributor.author |
Hopoglu, M |
|
dc.contributor.author |
Yakupogullari, Y |
|
dc.contributor.author |
Kizilkaya, C |
|
dc.contributor.author |
Kuzucu, C |
|
dc.contributor.author |
Isik, B |
|
dc.contributor.author |
Yilmaz, S |
|
dc.date.accessioned |
2022-03-30T13:21:48Z |
|
dc.date.available |
2022-03-30T13:21:48Z |
|
dc.date.issued |
2015 |
|
dc.identifier.uri |
http://hdl.handle.net/11616/59724 |
|
dc.description.abstract |
Background. Bloodstream infection (BSI) is an important concern in transplant patients. Early intervention with appropriate antimicrobial therapy is critical to better clinical outcome; however, there is significant delay when conventional identification methods are used. |
|
dc.description.abstract |
Methods. We aimed to determine the diagnostic performance of the FilmArray Blood Culture Identification Panel, a recently approved multiplex polymerase chain reaction assay detecting 24 BSI pathogens and 3 resistance genes, in comparison with the performances of conventional identification methods in liver transplant (LT) patients. A total of 52 defined sepsis episodes (signal-positive by blood culture systems) from 45 LT patients were prospectively studied. |
|
dc.description.abstract |
Results. The FilmArray successfully identified 37 of 39 (94.8%) bacterial and 3 of 3 (100%) yeast pathogens in a total of 42 samples with microbial growth, failing to detect only 2 of 39 (5.1%) bacterial pathogens that were not covered by the test panel. The FilmArray could also detect additional pathogens in 3 samples that had been reported as having monomicrobial growth, and it could detect Acinetobacter baumannii in 2 samples suspected of skin flora contamination. The remaining 8 blood cultures showing a positive signal but yielding no growth were also negative by this assay. Results of MecA, KPC, and VanA/B gene detection were in high accordance. The FilmArray produced results with significantly shorter turnaround times (1.33 versus 36.2, 23.6, and 19.5 h; P < .05) than standard identification methods, Vitek II, and Vitek MS, respectively. |
|
dc.description.abstract |
Conclusions. This study showed that the FilmArray appeared as a reliable alternative diagnostic method with the potential to mitigate problems with protracted diagnosis of the BSI pathogens in LT patients. |
|
dc.source |
TRANSPLANTATION PROCEEDINGS |
|
dc.title |
Rapid Detection of Bloodstream Pathogens in Liver Transplantation |
|
dc.title |
Patients With Film Array Multiplex Polymerase Chain Reaction Assays: |
|
dc.title |
Comparison With Conventional Methods |
|