dc.contributor.author |
Irtegun-Kandemir, S |
|
dc.contributor.author |
Icen-Taskin, I |
|
dc.contributor.author |
Bozkurt, M |
|
dc.contributor.author |
Kalkanli-Tas, S |
|
dc.date.accessioned |
2022-10-11T13:26:07Z |
|
dc.date.available |
2022-10-11T13:26:07Z |
|
dc.date.issued |
2019 |
|
dc.identifier.uri |
http://hdl.handle.net/11616/76230 |
|
dc.description.abstract |
Background: Behcet's Disease (BD) is a multisystemic inflammatory disorder affecting large vessels, lungs joints, gastrointestinal and neurological systems. The pathogenesis of BD remains poorly understood. Identifying the key signaling pathway is crucial for a complete understanding of the pathogenesis of BD. |
|
dc.description.abstract |
Objective: The aim of this study was to determine mRNA expression level of Src family kinases (SFKs) members and their involvement in lipopolysaccharide (LPS)-induced mitogen-activated protein kinases (MAPKs) regulation in peripheral blood mononuclear cells (PBMCs) of active BD patients. |
|
dc.description.abstract |
Methods: Twenty- five active BD patients and twenty-five healthy controls were included in the study. PBMCs were isolated from total blood by density gradient centrifugation. The mRNA expression levels of SFKs members were measured by real-time quantitative PCR (RT-qPCR). The effect of SFKs activity on LPS-induced activation MAPKs (Erk1/2, p38 and JNK) was examined by Western blot. |
|
dc.description.abstract |
Results: The mRNA expression levels of Hck, Src, Lyn, Yes and Fyn were found to be slightly decreased in active BD patients compared to the control subjects, but a slight change in mRNA level of SFKs members did not impact on protein levels and protein activity. LPS-induced Erk1/2 phosphorylation was significantly increased in the absence of SFKs activity in active BD patients. However, inhibition of SFKs activity had no effect on LPS-induced phosphorylation of p38 and JNK in both controls and active BD patients. |
|
dc.description.abstract |
Conclusion: SFKs downregulate LPS-induced Erk1/2 phosphorylation in PBMCs of active BD patients. |
|
dc.source |
ENDOCRINE METABOLIC & IMMUNE DISORDERS-DRUG TARGETS |
|
dc.title |
mRNA Expression Profile of SFKs and Involvement of SFKs in the |
|
dc.title |
Regulation of LPS-Induced Erk1/2 Signaling in PBMCs of Active BD |
|
dc.title |
Patients |
|